Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoAnika Erxleben avatar Anika Erxlebenorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoDelphine Lariviere avatar Delphine LariviereGuillaume Gautreau avatar Guillaume GautreauNathan Dunn avatar Nathan Dunnorcid logoErwan Corre avatar Erwan Correorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoBjörn Grüning avatar Björn Grüningorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoMaria Doyle avatar Maria DoyleValentin Loux avatar Valentin Louxorcid logoLaura Leroi avatar Laura LeroiNiall Beard avatar Niall BeardEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilSimon Bray avatar Simon BrayTwishi Gulati avatar Twishi Gulatiorcid logoAnna Syme avatar Anna Symeorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoCristóbal Gallardo avatar Cristóbal GallardoJasper Ouwerkerk avatar Jasper OuwerkerkAnton Nekrutenko avatar Anton Nekrutenkoorcid logoFelicitas Kindel avatar Felicitas KindelTorsten Seemann avatar Torsten SeemannHélène Chiapello avatar Hélène Chiapelloorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoHelena Rasche avatar Helena Rascheorcid logoJonathan Kreplak avatar Jonathan KreplakGildas Le Corguillé avatar Gildas Le Corguilléorcid logoBérénice Batut avatar Bérénice Batutorcid logoSimon Gladman avatar Simon Gladmanorcid logoMateo Boudet avatar Mateo Boudetorcid logoPaul Zierep avatar Paul ZierepBazante Sanders avatar Bazante Sandersorcid logoTeresa Müller avatar Teresa MüllerEkaterina Polkh avatar Ekaterina PolkhKenji Fujihara avatar Kenji Fujiharaorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoRomane LIBOUBAN avatar Romane LIBOUBANWilliam Durand avatar William Durandorcid logoAlexandre Cormier avatar Alexandre Cormier

Funding

These individuals or organisations provided funding support for the development of this resource